Milk and serum proteomes in subclinical and clinical mastitis in Simmental cows

Bovine mastitis causes changes in the milk and serum proteomes. Here both proteomes caused by naturally occurring subclinical clinical have been characterised quantified. Milk samples from healthy dairy cows (n = 10) were compared to those of with 12) using tandem mass tag (TMT) proteomics. Proteins that significantly increased or decreased 237) 117) quantified classified type change mastitis. A group proteins 38) showed a number which but milk, suggesting particular role host defence for maintaining restoring homeostasis during disease. affected bovine included coagulation pathways. Investigation modified was assessed assays haptoglobin, amyloid α1 acid glycoprotein validating results obtained quantitative Alteration abundance patterns proteins, together pathway analysis reveal multiple interactions related Data are available via ProteomeXchange identifier PXD022595. Mastitis is most serious condition affect leads reduced animal welfare as well having negative economic effect industry. Proteomics has previously identified mastitis, there few investigations addressing may blood infection. In this study, serum, proteomics approach cases investigated. individual determined into varying types altering abundance, such increasing showing no further increase Of special interest altered either similar trends - decreasing biological fluids reciprocal milk. As characterising potential markers severity disease, these provide insight pathophysiology response